Photoperiod regulates the daily profiles of Tryptophan Hydroxylase-2 gene expression the raphe nuclei of rats

Description

Tryptophan hydroxylase-2 mRNA (TPH, the rate limiting enzyme in 5-HT synthesis) expression levels display circadian variations in the median and dorsal raphe nuclei. This circadian pattern is under the control of the suprachiasmatic nuclei (SCN), the master clock. Photoperiodic cue is encoded by the SCN which convey the seasonal message to target sites. In the present study, we have investigated the effect of photoperiodic changes on the serotonergic neurones of the raphe nuclei. We have assessed the daily expression of TPH2 mRNA in both median and dorsal raphe nuclei of rats housed either under long photoperiod (18 h light/6 h dark cycle, LP18:6) or short photoperiod (SP6:18). Our results demonstrate that under LP18:6, TPH2 mRNA levels display a progressive decrease during the dark period and a maximal expression is reported at the beginning of the light period. The expression pattern of TPH2 mRNA under SP6:18 remains unchanged during the dark period and increases significantly before the day/night transition. This latter expression pattern is in line with the daily profiles of TPH2 mRNA reported previously under standard lighting regimen (12 h light/12 h dark cycle). The present results suggest that TPH2 mRNA expression pattern within DR and MR is affected by photoperiod which might in turn affect TPH content and 5­HT release within the circadian structures, but also in all the serotonergic projection areas of the brain.